Question

A spectrophotometer measures the transmittance, the absorbance, or both, of a particular wavelength of light after it has passed through a liquid sample. Before the transmittance or absorbance of the sample is measured, a cuvette filled only with solvent, called the blank, is placed in the spectrophotometer. Select the step that is not necessary to complete before a cuvette is placed into the spectrophotometer. Select one: a. Make sure the cuvette has exactly the same amount of solution so the readings are consistent. b. Make sure the cuvette is oriented in the spectrophotometer in the same way each time it is used. c. Wipe off the outside of the cuvette with a cleaning tissue to remove any debris or fingerprints. d. Rinse the cuvette with the sample solution, after washing, to remove residual solvent.

Answer 1

D

Step-by-step explanation:

Rinse the cuvette with the sample solution, after washing, to remove residual solvent.