Question

Fill In The Blank. In three separate site-directed mutagenesis experiments, scientists altered a Lys residue in corn PEP carboxylase to an Asn, Glu, or Arg. The substitution that you would expect to have the least effect on enzymatic activity would be ______ because it, like Lys, is ______ charged at physiological pH. The substitution that you would expect to have the most effect on enzymatic activity would be ______ because it is _____ charged at physiological pH. (Use structure tables if you need to.)

Answer 1

1. The substition that least affect on the enzymatic activity would be (arginine) because lysine is( positively) charged at physiological ph.

The substitution that have effect most on the enzymatic activity would be (Glu) because it is (negatively) charged at physiological pH.

Step-by-step explanation:

Fill In The Blank. In three separate site-directed mutagenesis experiments, scientists altered a Lys residue in corn PEP carboxylase to an Asn, Glu, or Arg. The substitution that you would expect to have the least effect on enzymatic activity would be ARGININE because it, like Lys, is POSITIVELY charged at physiological pH. The substitution that you would expect to have the most effect on enzymatic activity would be GLU because it is NEGATIVELY charged at physiological pH. (Use structure tables if you need to.)