Question

Plaque assays are often used to estimate the number of virions in a sample of a particular volume (the titer). The count is given as plaque-forming units. Which of the following is NOT generally a concern that must be considered in evaluating the results of plaque assays?

Answer 1

The largest problem is that single viruses may create multiple plaques, resulting on overestimates of the number of viruses present.

Step-by-step explanation:

Plague assay allows to measure the concentration of viruses in a sample, it is a widely used approach for determining the quantity of infectious virus. Therefore the amount of plaques created by a single virus is not a concern when evaluating the results of plaque assays.