Question

You have a mixture of three proteins X, Y, and Z.

a. X is positively charged, has a mass of 100 kDa, it binds sugars.
b. Y is positively charged, has a mass of 200 kDa, it does not bind sugars.
c. Z is negatively charged, has a mass of 100 kDa, it does not bind sugars.

What will be the most efficient method to purify protein X from the mixture of X, Y, and Z?

i. washing
ii. drying
iii. ion-exchange chromatography
iv. gel filtration chromatography
v. affinity chromatography

Answer 1

iii. ion-exchange chromatography

Step-by-step explanation:

We have to purify the proteins and column chromatography is used widely for the purification of proteins. Ion-Exchange chromatography is also a type of column chromatography.

When the sugar binding protein X passes through the column, the sugar and proteins binds together by exchanging ions and coagulated out no matter what the nature of charge on proteins...