Question

A specific steroid has a λmax of 282 nm and a molar absorptivity ε of 11 , 500 L mol − 1 cm − 1 . What is the concentration of the compound in a solution whose absorbance at 282 nm is A = 0.060 with a sample pathlength of 1.00 cm ?

Answer 1

The molar concentration of the compound is 5.2 x 10⁻⁶ M

Step-by-step explanation:

According to Beer-Lambert law the concentration of the sample and the sample path length have a linear relationship with the light absorbed at a particular wavelength.

This problem can be solved by this equation, which is as follows,

A = ε.b.C

Here, A is the absorbance, ε is the molar absorptivity, b is the sample path length and C is the molar concentration

0.060 = 11500 x (1) x C

C = 5.2 x 10⁻⁶ M