Answer: C4 and CAM
C4 -separated physically with the bundle sheath
CAM- with day stomata closure
Step-by-step explanation:
Theses two pathways were designed to ensure mximum C02 concentration, to avoid competition of their Phosphoenolphytuvate(PEP) substrate with competitor oxygen and to conserve water(CAM).
To achieve this the CAM plants e.g cacti.,orchids which thrive in extreme conditions, opens their Stomata at nights. therefore they take in enough Carbondioxide. This react with 3C PEP to from 4C- Oxaloaceteate. The reaction is catalyse by enzyme PEP carboxylase. The 4C oxaloacetate formed is converted to Malate by NAD+ Malase dyhydrogenase. The Malate is stored as Mailic acid in the vacuole.
During the day, the Stomata are closed, to conserve water, to prevent entry of oxygen the competitor with C02 for enzyme Rubisco.The ATP and NADPH, produced during the day initiated the process of Calvin Cycle. The Malate is transferred to the chloroplast and the stored C02 is removed by splitting Malate to pyruvate and CO2 by Malic enzymes. The C02 is pick up by enzyme Rubisco. It combines with Rubulose biphosphate for the Calvin Cycle to progress normally.
Thus C02 and Calvin cycle are separated in time
However the anatomy of C4 plants ensures separation of C02 from the enzyme Rubisco,by bundle shealth cells so that the freely diffused oxygen with C02 will not compete with C02 for enzymes Rubisco., for photorespiration to occurs, As mentioned above the C02 is conserved as 4C Malate, and then splits to C02 and pyruvate as in CAM.
However instead of the C02 diffusion into the chloroplast as in CAM plants, it is passed through into the air through group of air resistant bundle sheaths cells. Here it combines with PEP , and catalysed by enzyme Rubisco, for the normal Calvin Cycle process to continue.
Thus C02 captures and Calvin cycle stages are separated by physical barriers the bundle sheath.