Question

A binding protein binds to a ligand, L, with a Kd of 400 nM. How much ligand (in nM) is present when the fraction of ligand bound is 60%?

Answer 1

600nM

Step-by-step explanation:

• From Concentration of Ligand = Ykd/1 - Y

• where Kd = 400nM.

• fraction of ligand bound Y = 0.60

• Plugging the values of Y and Kd;

• Concentration = 0.6 x 400 / 1 - 0.6

• Concentration = 600 x 10^-9

• = 600nM