Question

The following peptides are subjected to normal electrophoretic analysis at pH 6.0. State whether the peptides will migrate towards the cathode or anode and predict the relative rate of migration of each peptide. a.GlyArg Phe.b.Gly.Arg Phe.c.Glu.Glu Phe.d.GIy.Glu

Answer 1

The peptide will definitely migrated towards cathode (negative terminal)

Step-by-step explanation:

The positively and negatively charged side chains of proteins cause them to behave like amino acids in an electrical field; that is, they migrate during electrophoresis at low pH values to the cathode (negative terminal) and at high pH values to the anode (positive terminal). The isoelectric point, the pH value at which the protein molecule does not migrate, is in the range of pH 5 to 7 for many proteins.