Question

In Southern blot hybridization, a labeled probe DNA is applied to a population of DNA molecules that have been separated by size using gel electrophoresis. The goal of this procedure is to:_______

Answer 1

Answer: The goal of this procedure is to identify the fractionated DNA that would be complimentary to the labelled probe DNA.

Step-by-step explanation:

The labelled probe DNA contains an already known specific sequence of bases that would be observed if it is complementary to the population of seperated DNA molecules. If it is, then a hybridization of the complimentary DNA sequence occur.

Thus, the goal of this procedure is to identify the fractionated DNA that would be complimentary to the labelled probe DNA.

Answer 2

Answer: To find out specific DNA of interest.

Explanation: By this technique; mutation in DNA can be detected, restriction site can be determined, single strand after separation can be analyzed, genetic disorders can be identified, infections can be identified.

This procedure is also helpful in the forensic.