Question

You want to know the exact location of a protein inside the cell. You know the gene sequence of the protein. There are antibodies available for this protein. Describe any one method you can use to visualize this protein.

Answer 1

Fluorescent Staining Method

Step-by-step explanation:

Fluorescent staining uniformly stains protein, is rapid and offers sensitivity equal to that attained by silver staining method. It involves no destaining step and is compatible with mass spectrometry and microsequencing. Commercially fluorescent stains are available to stain 1D or 2D gels to visualize bands.

Answer 2

Answer: Western Blotting

Explanation:

The sample is first denatured followed by the gel electrophoresis. Animal derived or synthetic antibody is produced which has the ability to bind to the protein.

The electrophoresis membrane is then washed by primary antibody before the excess antibody is washed off.

Then the secondary antibody is added to get attached to the primary antibody and then it can be visualized by immunofluorescence or some other method.