Answer:
2. The pathogen must be isolated from inoculated animals and must be different from the original organism.
Step-by-step explanation:
Koch postulates says that every disease is caused by a pathogen. That pathogen can be isolated from the host and can be grown in pure culture. If this pure culture is inoculated into a healthy host than it must cause the disease in that host. The same pathogen can be again isolated from the second host.
Therefore according to Koch's postulated the pathogen must be isolated from the inoculated animals and must be the same as the original organism therefore the second option is wrong.