Question

When using oil immersion you must start focusing and centering the specimen as usual with the 4X objective, then sequentially with the 10X, and 40X, and finally the 100X objectives.

At what point is the oil introduced?

Answer 1

Answer:The point at which oil is introduced is when focusing with 100X objectives.

Step-by-step explanation:

Oil immersion in microscopy involves the use of a highly magnification( such as 100X objectives) and a transparent oil which aims at increasing the amount of light that is passing through the mounted specimen at a short focal length for a better view of the specimen features. The purpose of using oil immersion technique is to reduce the amount of scattered light as magnification is increased; thus, at lower magnification( such as X10,X40) this is not noticed.

Answer 2

100X

Step-by-step explanation:

Majorly when using lower magnification microscope objective lenses (4x, 10x, 40x) the light refraction is not usually observed. But, at the 100x objective lens, the light refraction when employing a dry lens is observed. At a reducd light refraction, more light goes in the microscope slide and channelled at the very narrow diameter of a greater power objective lens. In microscopy, the greater the light = clear and vivid images. Immersion oil that has a refractive index same as the glass slide in the region filled with air, channels more light through the objective and a clear and crispy image is produced.

NOTE: OIL WILL DAMAGE THE OTHER LENSES.