Answer: 1:5 dilution = 1/5 dilution = 1 part sample and 4 parts diluent in a total of 5 parts. If you need 10 ml, final volume, then you need 1/5 of 10 ml = 2 ml sample. ... What is the DNA concentration of a 50 µl solution which contains 10 µl of DNA ... 200 ml final volume - 50 ml initial volume of 12x TBE = 150 ml water