Question

The double-reciprocal transformation of the Michaelis-Menten equation, also called the Lineweaver-Burk plot, is given by

1/V0= KM/(Vmax[S]) + 1/Vmax

To determine Km from a double-reciprocal plot, you would:

a. multiply the reciprocal of the x-axis intercept by –1.

b. multiply the reciprocal of the y-axis intercept by –1.

c. take the reciprocal of the x-axis intercept.

d. take the reciprocal of the y-axis intercept.

e. take the x-axis intercept, where V0= 1/2 Vmax.

Answer 1

To determine Km from a Lineweaver-Burk plot, multiply the reciprocal of the x-axis intercept by -1.

Step-by-step explanation:

To determine the Michaelis constant (Km) from a double-reciprocal plot or Lineweaver-Burk plot, you need to focus on the x-axis intercept. According to the Lineweaver-Burk equation:

1/V0 = (Km/Vmax)(1/[S]) + (1/Vmax)

Where V0 is the initial velocity, Vmax is the maximum velocity, [S] is the substrate concentration, and Km is the Michaelis-Menten constant.

To find Km, you should take the reciprocal of the x-axis intercept and multiply it by -1. This is because the x-axis intercept represents -1/Km in the Lineweaver-Burk plot. Therefore, the correct answer is:

• a. multiply the reciprocal of the x-axis intercept by -1.

Answer 2

option a

Step-by-step explanation:

Lineweaver–Burk plot also known as double displacement plot is used for the study of enzyme kinetics.

It is reciprocal of Michaelis-Menten equation. The Michaelis-Menten equation for enzyme catalysis is as follows:

`V=(V_(max) [S])/(K_m+[S])`

Take the reciprocal

`(1)/(V) =(K_m+[S])/(V_(max)[S]) =(K_m)/(V_(max)) (1)/([S]) +(1)/(V_(max))`

The plot or graph between 1/V and 1/[S] is called Lineweaver–Burk plot.

Slope of the plot is
`(K_m)/(V_(max))`.

Intercept of y-axis is .

Intercept of x-axis is
`-(1)/(K_m)`

Therefore, by taking the reciprocal of intercept of x-axis and multiplying by -1, Km value can be determined.

Therefore, the correct option is a.