Question

To produce a terminator nucleotide in Sanger DNA sequencing, a(n) ____ atom is removed from the hydroxyl group on the deoxyribose sugar where it bonds with the adjacent phosphate group

Answer 1

To produce a terminator nucleotide in Sanger DNA sequencing, a(n) oxygen atom is removed from the hydroxyl group on the deoxyribose sugar where it bonds with the adjacent phosphate group

Step-by-step explanation:

DNA consists of a double helix made up of nucleotides.These nucleotides are arranged in such a way that each nucleotide has a phosphate group , a sugar and a nitrogenous base.

Sanger sequencing is a method of DNA sequencing which is actually based on the selective incorporation of the chain-terminating dideoxynucleotides by DNA polymerase during the DNA replication

The Sanger sequencing has following steps :

1) denature dsDNA using heat.

2) Make multiple copies of a segment.

3) Attach a primer.

4) Add to four polymerase solutions.

5) Grow complementary chains until termination dye.

6) Denature the grown chains.

7) Electrophorese the four solutions.