Question

Each of the following statements describes a type of column chromatography. Sort the statements to the type of chromatography they describe. If a statement can describe all of the types, place that statement in the "All" category. (Note that size-exclusion chromatography may also be called gel-filtration or molecular exclusion chromatography.)

Categories: Size-exclusion Chromatography, Affinity Chromatography, Ion-exchange Chromatogrpahy, and All

Statements:
Separate molecules by size.
Separate molecules by charge.
The stationary phase has a covalently bound group to which a protein in the mobile phase can bind.
Uses a mobile phase and a stationary phase to separate proteins.
The stationary phase contains cross-linked polymers with different pore sizes.
Can separate molecules based on protein-lignand binding.
The stationary phase may contain negatively or positively charged groups.

Answer 1

Step-by-step explanation:

• Separate molecules by size. > All

• Separate molecules by charge. > Ion-exchange Chromatography, Affinity Chromatography

• The stationary phase has a covalently bound group to which a protein in the mobile phase can bind. > All

• Uses a mobile phase and a stationary phase to separate proteins. > All

• The stationary phase contains cross-linked polymers with different pore sizes. > Size-exclusion Chromatography

• Can separate molecules based on protein-ligand binding. > Affinity Chromatography

• The stationary phase may contain negatively or positively charged groups. > Ion-exchange Chromatography, Affinity Chromatography