Question

In phase-contrast microscopy, the differences in refractive indices between organisms and their environments are utilized for better viewing of living specimens.

True / False.

Answer 1

True

Step-by-step explanation:

In phase-contrast objective and a phase contrast optical systems is equipped with Light microscope .

Phase contrast microscope is used to differentiate unstained structures in a cell that differ in refractive indices and thickness. Thus it is usefull for examining living unstained organisms.

Light that passed through materials of different refractive index and/or thickness will undergo a change in Phase. The difference in phase or wave front irregularities cause variation in brightness of the structures.

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