Question

Researchers investigated the effect of urea on the three-dimensional structure of a certain enzyme. The researchers dissolved the enzyme in an aqueous buffer solution and added urea to the solution. The enzyme did not appear to have a secondary or tertiary structure. The researchers carefully removed the urea from the solution and determined that the enzyme had the original secondary and tertiary structure again.Based on the results of the experiment, which of the following statements best predicts the effect of urea on the enzyme’s function?1. Function will be disrupted by adding the urea and regained by removing the urea.2. Function will be disrupted by adding the urea, but it will not be regained by removing the urea.3. Function will be gained by adding the urea and disrupted by removing the urea.4. Function will be unaffected by the addition and removal of the urea.

Answer 1

A. Function will be disrupted by adding the urea and regained by removing the urea.

Step-by-step explanation:

Based on the information, adding the urea denatures the enzyme, which will disrupt the enzyme’s function by disrupting the active site. In contrast, removing the urea causes the enzyme to refold, which will restore the active site and the enzyme’s function.

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Answer 2

Function will be disrupted by adding the urea and regained by removing the urea.

Step-by-step explanation:

The protein is one of the important biomolecule made of the polymers of the alpha amino acids that are linked together by peptide bonds. The protein works at their specific pH.

Denaturants disrupt the 3 dimensional structure of protein that affect their normal functioning. In the given experiment, the urea acts as denaturant and disrupt the protein structure. The removal of urea will reverse the denaturant condition and protein gain its function.

Thus, the correct answer is option (a).