Question

Pancreatic cells, which secrete a large amount of digestive enzymes, are labeled with radioactive leucine and then chased for several hours with nonradioactive leucine. Photographic emulsions are prepared at different times during the chase. Where would the black spots appear on an emulsion prepared 3 hours after the pulse?a. Secretory vesicles

b. Rough ER
c. Exterior of the cell
d. Golgi apparatus

Answer 1

Exterior of the cell.

Step-by-step explanation:

The pulse chase experiment was done to understand the secretion pathway of the protein at different time intervals. The protein travels through the endomembrane system for its secretion.

The cells of the pancreas are labelled with the leucine which is radioactive in nature. The black spots will be shown on the cell exterior as three hours are enough for the secretion of the protein outside the cell. This indicates that the protein has been secreted out of the cell.

Thus, the answer is exterior of the cell.