Question

Which of the following is not part of the normal process of cloning recombinant DNA in a bacterial host cell?

a. Restriction endonuclease digestion of plasmid DNAâs
b. Production of new recombinant DNA using DNA ligase and digested plasmid DNAs
c. Joining of the target DNA and the plasmid cloning vector in order to create a newly synthesized DNA
d. Transformation of bacterial host cells by the recombinant DNA plasmids and selection using kanamycin
e. Production of mRNA from the cloned gene
f. None of the above

Answer 1

e. Production of mRNA from the cloned gene

Step-by-step explanation:

During cloning in a bacterial host the recombinant DNA is replicated but not necessarily transcribed to mRNA. The promoters are controlled so that trancription and translation of the gene may occur under certain conditions. For example addition of an inducer such as IPTG into bacterial media inducing protein expression via promoter binding.