Question

Which of the following would you find in a Sanger sequencing reaction but not in a polymerase chain reaction? 1. DNA polymerase 2. ddNTPs 3. DNA template 4. DNA primer 5. dNTPs

Answer 1

2. polymerase chain reaction

Step-by-step explanation:

In Sanger's method, the process is used to make copies of unknown DNA sequences with different lengths/fragments using ddNTPs by chain termination method.

On the other hand, in polymerase chain reaction (PCR) complete copies of gene clones are required. Thus, there is no need of fragment formation so that it does not require ddNTPs.