Question

A DNA template is composed of the following nucleotide sequence: 5'-TACGCATT-3' What is the mRNA transcript for this template?

A. 5'-TTACGCAT-3'
B. 5'-AAUGCGUA-3'
C. 5'-UACGCAUU-3'
D. 5'-AATGCGTA-3'
E. 5'-AUGCGUAA-3'

Answer 1

The mRNA transcript synthesized from the DNA template sequence 5'-TACGCATT-3' would be 5'-AUGCGUAA-3', corresponding to option E.

Step-by-step explanation:

The student's question pertains to the transcription process in molecular biology, where a DNA template is used to synthesize a complementary mRNA strand. Given the DNA template sequence 5'-TACGCATT-3', transcription involves matching the DNA nucleotides with their RNA complements: adenine (A) with uracil (U), thymine (T) with adenine (A), cytosine (C) with guanine (G), and guanine (G) with cytosine (C), respecting the antiparallel directionality. Utilizing these base-pairing rules, the mRNA sequence transcribed from this DNA template is 5'-AUGCGUAA-3', which matches with option E from the given choices.

Answer 2

E. 5'-AUGCGUAA-3'

Step-by-step explanation:

The nucleotide sequence of the template strand specifies the nucleotide sequence of mRNA. Adenine in template strand pairs with uracil base in mRNA, thymine with adenine, guanine with cytosine and cytosine with guanine base. Also, the polarity of the DNA template strand and that of the mRNA is anti-parallel which means that 3' end of the template strand is present opposite to the 5' end of the mRNA. Therefore, a DNA template strand with nucleotide sequence 5'-TACGCATT-3' would code for the following mRNA:

DNA template strand: 5'-TACGCATT-3'

mRNA strand: 3'-AUGCGUAA-5'