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What is the best explanation for the observed behavior of the E147D variant? The substitution results in:

User Jezdez
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1 Answer

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Answer:

A repulsive interaction that reduces conformational stability and thus enzymatic activity.

Step-by-step explanation:

The reason behind this repulsive interaction that leads to reduction of the conformational stability and thus the enzymatic activity is that aspirates has a shorter chain even though both glutamate and aspirate are carboxylic acid. The short chain of aspirate is therefore at a distance from the active site and closer to D348 which leads to repulsive interaction hence reducing conformational stability and enzymatic activity.

User Dbr
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