235k views
0 votes
How is modern day dna profiling different from the original dna fingerprinting process?

2 Answers

1 vote

The new DNA profiling technique differs from the initial DNA fingerprinting system because it does not use DNA cutting restriction enzymes. Instead of restriction enzymes in DNA profiling, polymerase chain reaction is applied to break the DNA. It is a delicate technique. In paternity checks and even to solve court crimes, this approach is used. It is used on the root of the scalp, a small volume of saliva or blood, skin cells. In the case of the DNA fingerprinting process, restriction enzymes are used. Precision depends on the number of markers. If the number of markers is high, it would also be high in precision. It's an expensive operation here. Thirteen autosomal STR markers are needed, along with data of 10 alleles maximum, according to the national DNA index system.

User ThuyNguyen
by
4.0k points
2 votes

Answer:

The modern day DNA profiling is different from the original DNA fingerprinting process is that it does not use the restriction enzymes to cut DNA.

Step-by-step explanation:

Polymerase chain reaction is applied to break the DNA instead of the restriction enzymes in the DNA profiling. It is a sensitive method. This method used in paternity test and also for solving criminal cases. This is used on the hair root , skin cell , small amount of saliva or blood.

Restriction enzymes is used in case of the DNA fingerprinting method. The accuracy depends upon the number of markers. If the number of markers are high, then the accuracy will be high too. This is a costly process. According to the nation DNA index system, 13 autosomal STR markers are needed, along with information of 10 loci maximum

User Catalin Hritcu
by
4.5k points