Question

Phosphofructokinase, one of the enzymes which regulates the glycolysis pathway, is activated by ___________.

Answer 1

Fructose‐2,6‐ bisphosphate

Step-by-step explanation:

Phosphofructokinase is activated by fructose‐2,6‐ bisphosphate, which is formed from fructose‐1‐phosphate catalyzed by phosphofructokinase II. Thus, the abundance of F6P results in a higher concentration of fructose 2,6-bisphosphate (F-2,6-BP) and hence activated more phosphofructokinase enzyme and its activity.

Answer 2

The answer is fructose 2,6-bisphosphate

Step-by-step explanation:

Phosphofructokinase is activated by a high concentration of AMP, but the most potent activator is fructose 2,6-bisphosphate, which is also produced from fructose-6-phosphate by PFK2.

Fructose 2,6-bisphosphate activates phosphofructokinase by increasing its inclination towards fructose 6-phosphate and reducing the inhibitory effect of ATP. In essence, Fructose 2,6-bisphosphate is an allosteric activator that shifts the conformational equilibrium of this enzyme from the T state to the R state.