Answer:
The sequence of codons in mRNA is most directly responsible for the sequence of amino acids in a protein.
Step-by-step explanation:
During translation, mRNA is read in the 5´ to 3´ direction, and tRNA transfers amino acids to build the polypeptide chain. The protein is synthesized from the amino terminus to the carboxy terminus. Each amino acid is specified by a codon formed by three bases in the mRNA.
A codon is a sequence of three nucleotides that belongs to a specific amino acid. There are 64 different combinations of three nucleotids, 61 of them are amino acid-specific and the other three are used as a stop signal.
mARNs have a start and end codon that tells the ribosome where to start and stop adding amino acids. When the ribosome reaches the end codon, it means that protein synthesis is finished.