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What made the first difference in the colonies

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Answer:

In 1606, King James I of England granted charters to both the Plymouth Company and the London Company for the purpose of establishing permanent settlements in America. The London Company established the Colony and Dominion of Virginia in 1607, the first permanently settled English colony on the continent. The Plymouth Company founded the Popham Colony on the Kennebec River, but it was short-lived.

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