The Michaelis-Menten and the Lineweaver-Burk plots can be used to determine the Km and Vmax of the enzyme-catalysed reaction. Explain the significance of Km value in enzyme catalysed reaction. By using succinate dehydrogenase as the enzyme, sketch a Lineweaver-Burk plot that shows an enzyme catalysed with and without competitive inhibitor. Explain what will happen if malonate is added to the reaction. Relate the Lineweaver-Burk plot to the effect of malonate on Km and I'max values. Using the knowledge from cell biology, explain how the enzyme is being synthesis.