Question

Important steps in sequencing pure proteins include all of these except: a. Determining the amino acids on the ends of the smaller peptides b. Determining the isoionic pH of the protein c. Determining the amino acids on the ends of the protein d. Breaking the protein into smaller peptides e. Determining the amino acid composition

Answer 1

b. Determining the isoionic pH of the protein

Step-by-step explanation:

The sequencing of pure proteins is nothing more than the analysis of the amino acids present in isolated proteins. This analysis is composed by the study of the sequence that the amino acids assume within the protein, the amino acids present in the extremities and the constitution of each one of these amino acids. In summary, we can state that protein sequencing refers to the constitution of these proteins. In this case, the isotonic pH of these proteins is not relevant information.