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Explain the steps you would follow to make 500 mL of a 0.3 M solution of sucrose

(C12H22011) in the lab.

1 Answer

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Answer:

see explanation

Step-by-step explanation:

Solutions are prepared from one of three solute sources ...

- solid solute from manufacturer,

-liquid solute from manufacturer,

-stock concentrate of solute for dilution to lower concentrations.

In this problem, sucrose is a stock solid with a formula mass of 342.3 grams/mol. The amount of solid needed can be determined using the formula:

mass of solute needed (grams) = (Molarity needed x Volume needed in Liters x formula weight) / (decimal fraction purity of stock solid)

Molarity needed = 0.3M

Volume needed = 500 ml = 0.500 liters

Formula weight = 342.3 grams/mole

Purity factor (assumed) = 100% = 1.00 (should be posted on stock bottle label) For example, a 95% pure stock solid => purity factor of 0.95.

∴ grams of sucrose needed = (0.3M*)(0.500L)(342.3g/mole)/(1.00) =51.345 grams sucrose.

Measure 51.345 grams of sucrose into mixing vessel and add solvent water up to, but not to exceed 500 ml total volume. Mix until homogeneous.

User Bruno Mello
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