Question

A sample from a stock of a bacterial colony in liquid media was diluted by a factor of 106, and 2 ml of this dilution was spread on a Petri dish of solidified media. 56 colonies were observed. What was the concentration of bacteria of the initial stock?

Answer 1

28 × 10⁶ colonies/ml

Step-by-step explanation:

Let C be the concentration of bacterial in the initial stock. When it is diluted by a factor of 10⁶, the new concentration is C' = C/10⁶.

When 2 ml of this concentration is spread on a Petri dish of solidified media, 56 colonies were produced. The number of colonies, n after spreading the 2 ml of C' is C' × 2 ml = 2C' = 2C/10⁶.

So, n = 2C/10⁶.

Since the number of colonies after spreading on a Petri dish of solidified media is 56, n = 56 colonies.

So, 2C/10⁶ = 56

Making C subject of the formula, we have

C = 56 × 10⁶/2

C = 28 × 10⁶ colonies/ml

So, the initial concentration of bacteria is 28 × 10⁶ colonies/ml