Question

Outline an experimental approach to determining the average chain growth rate for transcription in vivo. Chain growth rate is the number of nucleotides polymerized per minute per RNA chain. Fill in the blanks.

Label a growing bacterial or cell culture for a brief interval with a mixture of 14C-labeled nucleosides, which would be converted to nucleotides and incorporated into _____. Isolate total ______and subject it to hydrolysis with mild _____. Every _____-terminal nucleotide will be released as a nucleoside. Separate nucleosides from nucleotides, by ______. Total picomoles of nucleotide in the hydrolysate divided by total picomoles of nucleoside gives the number of nucleotides incorporated per _______chain-which, when divided by the labeling interval (minutes) gives the growth rate of individual chains (nucleotides incorporated per ______ chain per minute). An interesting variant of the experiment would be to carry it out with individual radiolabeled nucleosides and ask whether the chain ______is the same for each nucleotide.

Answer 1

DNA

Protein

temperature

nerve

esterification of inorganic phosphate to C5 on aldose.

nucleic acid polymer

growth rate

Step-by-step explanation:

Nucleosides have nitrogen base surrounded by 5 carbon carbohydrates which is called as ribose molecule. Nucleotides is simply a nucleoside with one or more phosphate group. There are 4 different types of nucleosides which are, adenosine, guanosine cytidine, and uridine. Nucleotides are formed from nucleosides by esterification of inorganic phosphate on aldose.