Question

Which technique is best suited to each application?

a. In the second week of a four week biochemistry experiement, you have 50 fractions collected from a gel filtration column to determine which fractions contain lactate dehydrogenase. You are given only 400 uL of 0.100 mg/mL lactate dehydrogenase to prepare your calibration curve. 96-well microplate
b. Your environmental lab has 2000 samples to be analyzed for trace ammonia by next week. discrete analyzer.
c. Twenty water samples must be analyzed for Cl-, NH3, PO3-, and So during each work shift. flow injection analysis colorimeter.
d. Your professor heard you will be hiking the Appalachian Trail next summer. She asks you to collect 100-mL water samples from the ten streams with the highest concentration of phosphate.

Answer 1

a. discrete analyzer

b. 96 well microplate

c. flow injection analysis

d. colorimeter

Step-by-step explanation:

96 well microplates are instruments designed for sample collection and throughput screening. If an environment lab has collected 2000 samples then 96 well microplate is best suited application. Discrete analyzer is automated chemical analyzer which performs test on samples kept in discrete cells. Flow injection analysis is approach used for chemical analysis. It injects a plug of sample into a flowing carrier stream. Colorimeter is a device which measures absorbance of wavelength of light by a specific solution.