Answer:
Classical chemical methods, enzymatic assays, gas chromatography (GC), liquid chromatography (LC), and mass spectrometry (MS)
Step-by-step explanation:
Classical chemical methods, enzymatic assays, gas chromatography (GC), liquid chromatography (LC), and mass spectrometry (MS) are the alternate methods used for the detection of cholesterol. These methods are used when HRP would not be commercially available for the analysis or detection of cholesterol in the patient's body so these methods can be used for the purpose of detection of cholesterol.