Given 200ul of a 0.5mg/ml stock solution of BSA, how much do you pipet into a test tube so that you are adding 5ug of BSA to the test tube

Question

asked Nov 20, 2022 96.6k views

1 Answer

JCasso · Answer 1 · 2022-11-25T20:16:26+0000

Answer:
$10\mu L$ of volume needs to be pipetted out in the test tube.

Step-by-step explanation:

We are given:

Mass of BSA to be formed =
$5\mu g=0.005mg$ (Conversion factor:
$1mg=1000\mu g$

Volume of stock solution =
$200\mu L=0.2mL$ (Conversion factor:
$1mL=1000\mu L$

It is also given that for the mass of BSA is 0.5 g, the volume used up is 1 mL

In order to have, 0.005 g, the volume of stock solution needed will be =
$(1mL)/(0.5g)* 0.005g=0.01mL=10\mu L$

Hence,
$10\mu L$ of volume needs to be pipetted out in the test tube.