Hi. Your question is incomplete. Unfortunately you did not submit the experiment to which this question refers, which makes it impossible for your question to be answered. However, I will try to help you as best I can.
It is likely that the scientist in this experiment decided to compare the DNA of the wolves presented in the question above, because he wanted to evaluate how the variables in this experiment acted in relation to the different treatments. This comparison is the basis for the scientist to determine the results of the experiment and to assess the differences in the experimental treatments.
In that case, to answer this question, you must read your experiment and identify the variables and treatments, and determine how these wolves fit into each treatment.
In an experiment, the treatments are the groups where the variables are tested, whereas the variables are the elements of interest to be studied.