Answer:
4) The mRNA was shorter because of Intron splicing
Step-by-step explanation:
In eukaryotic cells, the process of gene transcription uses a fragment of DNA as template in order to synthesize a complementary single-stranded RNA molecule known as primary messenger RNA transcript or 'pre-mRNA', which is then processed to yield a mature messenger RNA (mRNA). This mature mRNA is finally used as template to synthesize a polypeptide chain (i.e., a protein) by the process of translation. RNA splicing refers to the processing mechanism by which a pre-mRNA is transformed into a mature mRNA. During RNA splicing, non-coding regions of the pre-mRNA called 'introns' are removed, whereas the remaining coding regions called 'exons' are joined toghether to form a mature mRNA. In consequence, RNA splicing generates an mRNA that is shorter than the originally transcribed gene sequence.