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Which of the following BEST describes the template DNA for the positive control PCR tube in our Wolbachia PCR?

a. The positive control tube receives each kind of template DNA: genomic DNAs from insects known to be and predicted to be positive and negative for Wolbachia infection.
b. The positive control tube receives genomic DNA from an insect known to be positive for Wolbachia infection.
c. The positive control tube receives any genomic DNA from a student's sample that is predicted to be infected with Wolbachia.
d. The positive control tube receives water for template DNA.

User Sydwell
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Answer: c. The positive control tube receives any genomic DNA from a student's sample that is predicted to be infected with Wolbachia.

Step-by-step explanation:

Positive PCR control can be defined as the control sample that is used as a benchmark for comparison with the results of the PCR or polymerase chain reaction. It comprises of artificial genome or primers. For amplification of microbial DNA synthetic oligonucleotides are used as targets of each primer and a hydrolysis probe is also used. As it is already known that primer as well as hydrolysis probe has the specificity for the DNA strand so it will help in identification of the specific region or sequence in the DNA structure. Wolbachia DNA can be detected if the positive control consists of samples of students affected by Wolbachia that can give indication of whether the Wolbachia species is the same as that of the one which has caused infection.

User Sihaya
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