Question

A cell that utilizes NHEJ to repair a double-stranded DNA break which occurred in the open reading frame of a gene is susceptible to indels resulting in a frame shift mutation and ultimately a non-functional protein. False True

Answer 1

True

Step-by-step explanation:

The non-homologous end-joining (NHEJ) pathway is a DNA repair pathway used to repair double-strand breaks that can occur throughout all cell cycle phases. Conversely to the homologous recombination (HR) pathway, the NHEJ pathway does not need a homologous template at the DNA double-strand breaks ends and therefore NHEJ is an error-prone repair pathway that may cause mutations within the repaired region of the DNA. The NHEJ pathway is well-known to induce indels (i.e., insertions and deletions) mutations that shift the reading frame of the protein (i.e., frameshift mutations), thereby leading to a completely different translated protein from the original one.