Question

g (2pts) A 10x transfer buffer solution is 250mM Tris and 1.92M glycine. Buffers are always used at 1x concentration in the lab (unless specified otherwise in the protocol), so we will have to dilute the 10x buffer to 1x before use. What is the concentration of Tris and glycine in the 1x buffer

Answer 1

The explanation according to the given question is summarized below.

Step-by-step explanation:

Given:

Tris,

= 250 mM

Glycine,

= 1.92 M

According to the solution,

For the dilution pf 10X to 1X buffer, we get

=
`1 \ ml \ of \ 10X \ buffer +9 \ ml \ of \ distilled \ water`

=
`10`

i.e.,

⇒
`10X \ to \ 1X=1:10 \ dilution`

Now,

⇒
`10X (250 \ mM\ Tris \ HCl, 1.92M\ Glycine, and\ 1 \ percent ((w)/(v) ) SDS) ---->1X(25 \ mM \ Tris \ HCl,0.193 M\ Glycine, and \ 0.1 \ percent((w)/(v) )SDS)`