Question

In illumina nest generation sequencing the nucleotides are identified by

Answer 1

In Illumina's next-generation sequencing, nucleotides are identified using synthesis sequencing. The DNA sample is fragmented, modified with DNA adapters, and amplified by PCR. Each bead with a DNA fragment is put into a well with sequencing enzymes, and the detection of light flashes determines the order of nucleotides.

Step-by-step explanation:

In Illumina's next-generation sequencing, the nucleotides are identified by synthesis sequencing. In this process, the DNA sample is fragmented and modified with DNA adapters. Each fragment is immobilized on a bead and amplified by PCR, creating many copies of that DNA fragment on the bead. Each bead is then placed in a well with sequencing enzymes, and the four nucleotides are added one by one. When a nucleotide is incorporated, pyrophosphate is released, emitting a small flash of light that is recorded by a detector. This allows the order of nucleotides to be determined and the DNA sequence to be obtained.

Answer 2

Step 3 in NGS Workflow: Data Analysis

After sequencing, the instrument software identifies nucleotides (a process called base calling) and the predicted accuracy of those base calls. During data analysis, you can import your sequencing data into a standard analysis tool or set up your own pipeline.

Step-by-step explanation:

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