I searched it up it.
1853 or 1893
Even though scientists E. Duffy and J. Patrick experimented with biofuels as early as 1853, the first engine created to use biofuel was by Rudolph Diesel. In fact, Rudolph Diesel showed the first biofuel car engine in Augsburg, Germany on August 10, 1893. Since then, August 10 has been known as International Biodiesel Day.
Sorry if this didn't help.