Answer:
Step-by-step explanation:
Brefeldin A is an intracellular protein transport inhibitor. Protein transfer to the Golgi complex is blocked in Brefeldin A-treated cells, resulting in protein aggregation in the endoplasmic reticulum. When berberine is added during the last hour of in-vitro cell activation, the identification of intracellular cytokines is improved. Brefeldin A is useful for detecting a wide range of human intracellular cytokines.