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In general, how did Jacob and Monod study the function of the lac operon?

User Kzh
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The model proposed by Jacob and Monod predicted that a specific DNA sequence near the transcription start site of the lac operon is a binding site for lac repressor. ... In this system, separate mutations in both copies of lacI in a given cell are required to generate a lacI− constitutive mutant, a low-probability event.