Question

In whole-genome shotgun sequencing, the chromosome is cut into pieces, the pieces are sequenced, and the sequences are aligned based on overlapping sequences into a contig. Place the fragments of DNA in the proper places to form the contig for this sequence of DNA, using the central CAT sequence as a guide. ResetHelp CATCGAGCATCGAG CCAGGCCCCAGGCC GGCCATCGGCCATC AGGCCATAGGCCAT CCATCGACCATCGA

Answer 1

CCAGGCC

CCATCGA

GGCCATC

CAT

AGGCCAT

CATCGAG

Step-by-step explanation:

Shotgun sequencing is a method used to determine the nucleotide sequence of entire chromosomes/genomes. This sequencing method consists of obtaining random DNA fragments which are subsequently classified by bioinformatic tools that ordering them according to overlapping sequences called contigs. In the whole-genome shotgun (WGS) technique, the entire genome of an organism is sequenced, being the critical factor the depth of sequencing, which refers to the quality of the sequencing reads (e.g., a depth of 20X indicates that the genome is sequenced 20 times by a sequencing machine). For the human genome, WGS became available after the completion of the Human Genome Project (HGP), which enabled the generation of a reference sequence for the whole human genome. The steps of the WGS technique are the following:

1. Preparation of isolated chromosomes

2. The DNA is sheared into small fragments

3. The DNA fragments of about 1 kilobase (1000 base pairs) are incorporated into plasmids which are cloned to render pure samples of each DNA fragment

4. The plasmid clones are sequenced by sequencing machines

5. Bioinformatic tools finally are used to link DNA fragments by their overlapping ends