Question

What is the difference between traditional PCR and qPCR?

a. Traditional PCR has only 1 cycle whereas qPCR has multiple cycles
b. In traditional PCR, the presence or absences of a DNA sequence is analyzed upon reaction completion whereas qPCR monitors DNA amplification as the reaction progresses
c. Traditional PCR is an outdated technique which is unreliable and has not been utilized for years whereas qPCR is a newer and better technique which is always preferred nowadays.
d. Traditional PCR is monitored by an increases in fluorescence signal whereas it is impossible to monitor qPCR by increases in fluorescence signal

Answer 1

The difference between traditional PCR and qPCR (Real-time PCR) is:

b. In traditional PCR, the presence or absences of a DNA sequence is analyzed upon reaction completion whereas qPCR monitors DNA amplification as the reaction progresses

Step-by-step explanation:

Real-time PCR (qPCR) is an advanced tool for analyzing RNA and DNA. With Traditional PCR detection occurs at the end-point of the reaction, while with Real-time PCR, detection of the RNA and DNA sequencing is detected as the reaction is occurring. The advantages of the qPCR over the traditional PCR include higher precision and resolution, and increased dynamic range and sensitivity.

Polymerase Chain Reaction (PCR) is a widely-used Biology tool to rapidly replicate millions of copies of a specific DNA sample.