Answer:
by performing a bioinformatic analysis in order to discover Y chromosome-linked mutations associated with defective phenotypes. In this case, it is expected to discover two or more loss of function mutations located in the coding region of the target gene on the Y chromosome in different males, and then associate such mutations with altered phenotypes caused by the inactivation (loss of function) of this gene
Step-by-step explanation:
The genes located on the Y chromosome exhibit a uniparental mode of inheritance (i.e., these genes are only transmitted from father to their sons). This situation makes difficult to map the target gene since recombination does not occur for genes that are only located on the Y chromosome, hampering the development of a linkage map containing this gene. In this case, it is possible to directly associate genetic mutations located at the same genomic region (locus) on the Y chromosome with a particular altered phenotype in males carrying these mutations, which can be in principle associated with the inactivation of the target gene (loss of function mutations) in these individuals.