Final answer:
The correctly punctuated and capitalized sentence is 'Generally speaking, the ladies' Bible study lasts forty-five minutes,' due to proper use of the comma and possessive form, as well as correct capitalization of 'Bible.'
Step-by-step explanation:
The sentence that is punctuated and capitalized correctly is: Generally speaking, the ladies' Bible study lasts forty-five minutes. In this sentence, there's a correct use of the comma after the introductory phrase 'Generally speaking,' and proper possessive form 'ladies'' to show that the Bible study belongs to the ladies. Also, 'Bible study' is correctly capitalized as 'Bible' refers to the holy book, and such titles are always capitalized; 'study' doesn't need to be capitalized as it is a common noun in this context.