Question

The primers you used in lab amplified 145 base pairs that were adjacent, but not included in the repeat. If you used a different primer that only amplified 130 base pairs adjacent to a 14 base pair repeat, and you had 4 repeats in your product, what was the size of your final PCR product?

186

214

158

200

172

Answer 1

186

Step-by-step explanation:

The Polymerase Chain Reaction (PCR) is a technique used in molecular biology to amplify small segments of DNA. This technique uses a pair of oligonucleotide sequences called 'primers' which bind to opposite strands of the heat-denatured DNA template in order to amplify this target DNA sequence. In this case, the DNA template is composed of a single unit of 130 base pairs (bp) and four repetitive units of 14 bp, so the final PCR product will have 186 bp >>> 130 bp + (14 bp x 4) = 130 + 56 = 186.