So I’m a leave this for you to decide but here’s my point of view. if you say yes it would be because since they’re separated by a highway they won’t get to cross as much and and they’ll adapt differently because one side has a pond and the other one doesn’t but at same time if you say no that you shouldn’t categorize them because yes they are the same species but they could still cross the highway naked and still interbreed the highway just makes everything difficult
so you could either say yes you should classify them as different species because they were ultimately adapt differently or you can say no because even though there is a highway they can still cross is just much harder